Biotin

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Biotin Monograph

Introduction Biotin is a water-soluble B vitamin that is an essential cofactor for four carboxylase enzymes, each of which catalyzes an essential step in intermediary metabolism. Because humans and other mammals cannot synthesize biotin, it must be derived from dietary sources and de novo synthesis by intestinal bacteria. Biotin was originally recognized when rats fed protein derived from egg w...

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A biotin-streptavidin-biotin bridge dramatically enhances cell fusion

Although the generation of hybrid cells by cell fusion plays a significant role in biotechnology and biomedicine, the low cell-fusion rates and the limitation of large-scale cell fusion for clinical applications of the two widely used approaches, polyethylene-glycol (PEG)-mediated cell fusion and electrofusion, hinder the application of this critical technology in certain key areas, including c...

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Acute glucocorticoid treatment increases urinary biotin excretion and serum biotin.

Previous studies have demonstrated that glucocorticoids alter biotin metabolism. To extend these studies, the effect of dexamethasone on biotin pools was analyzed in rats consuming a purified diet containing a more physiological level of dietary biotin intake (0.06 mg/kg). Acute (5 h) dexamethasone administration (0.5 mg/kg) elicited elevated urinary glucose output as well as elevated urinary b...

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Structure and Complexation with Biotin and Biotin-Lipid Assemblies

Fourier-transform infrared studies have been carried out to investigate the secondary structure and thermal stability of hen egg white avidin and its complexes with biotin and with a biotinylated lipid derivative, N-biotinyl dimyristoyl phosphatidylethanolamine (DMBPE) in aqueous dispersion. Analysis of the amide I stretching band of avidin yielded a secondary structural content composed of app...

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Biotin biosynthetic enzymes and the metabolic control of biotin biosynthesis

Biotin is a vital cofactor for many enzymes that facilitate carboxylation, decarboxylation, and transcarboxylation reactions. The biotin biosynthetic network has been well characterized in many microorganisms including E.coli (Eisenberg, 1973), but only recently have the genetic and biochemical components of the Arabidopsis biotin biosynthetic enzymes been discovered. Previous studies (Muralla ...

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ژورنال

عنوان ژورنال: Advances in Nutrition

سال: 2012

ISSN: 2161-8313,2156-5376

DOI: 10.3945/an.111.001305